Genetically engineered crops are a key component of U.S. agriculture and new methods to
engineer traits of interest are emerging that have the potential to provide unparalleled
specificity in genome modification. However, quantitative data in crop species of potential off-target
effects in the genome following targeted genome modification are essential for informed
risk assessments of transgenic, cisgenic, and intragenic approaches to engineer plants. This
project is focused on collecting quantitative, whole genome sequence and expression data as
well as replicated field trial phenotypic data in a panel of potato lines engineered for herbicide
resistance using two distinct genome modification methods, CRISPR-Cas9 and TALENs.